Formal ether concentration technique 20142015 abstract. Formol ether concentration technique was used to analyze the stool samples. Formalin acts both a fixative and preservative of protozoan eggs, larvae and cysts. Fecal debris is extracted into the ether phase so that the ethyl acetate formalin 510% nss. Low sensitivity of the formolethyl acetate sedimentation. Formol ether concentration technique was used to concentrate the gut content and analysis carried out. The most commonly used fixatives for stool preservation are either formalin or sodium acetateacetic acidformalin saf 3, 24, 40. They showed that the formol ether concentration technique was more sensitive as compared to the other methods. Parasitic elements are concentrated through sedimentation to enhance the recovery in fecal specimens.
Diagnostic performance of direct wet mount microscopy in. The technique recommended for the qualitative diagnosis of intestinal parasites both helminths and intestinal protozoa is the formol ether concentration method fecm 7,8, 9 performed on three. Adaptation of ritchies method for parasites diagnosing. The bottle was capped and mixed by shaking for about 20 seconds. According to ritchie 1948, the recovery efficiency of the mgl technique for. In such cases, the use of concentration techniques increases the chances of detecting parasitic organisms, thus increasing the sensitivity of copromicroscopic techniques. Article pdf available in journal of clinical pathology 236. The formol ether diagnostic technique was generally more sensitive with s. Modified formalinether concentration technique for.
Miranda questions for research update brief summary draw and label. A total of 103 samples collected from the same number of individuals were examined for intestinal parasites using the direct smear method and the formol ether concentration technique. After stirring well, more salt solution was added till the container was. The helminth parasite community of european badgers meles. Modified formalinether concentration technique for diagnosis. An estimated peasize of faeces was emulsified in 4 ml of 10% formol water.
Stool specimens were collected and examined using formol ether concentration technique. Prevalence and associated factors of intestinal parasitic. Comparison of sensitivity and specificity of 4 methods for. Get a printable copy pdf file of the complete article 319k, or click on a page image below to browse page by page. With the different solutions added, fecal debris is extracted into the ether phase and parasitic elements are sedimented at the bottom. The technique recommended for the qualitative diagnosis of intestinal parasites both helminths and intestinal protozoa is the formol ether concentration method fecm, performed on three different samples, but the direct fecal smear on a single sample is used as diagnostic method more often in resourceconstrained settings.
Pdf teknik konsentrasi formol eter untuk mendiagnosa. Comparison of modified 1% potassium hydroxide formol ether concentration technique with direct wet mount preparation and standard formol ether concentration technique for detection of parasites in. Sedimentation techniques use solutions of lower specific gravity. Disadvantagesoftheformalin ether technique include i concentration ofgranular material, ii suboptimal concentration of h. Excreta disposal facilities and intestinal parasitism in. The specific gravity of protozoan cysts and helminth eggs is greater than that of water. Pdf comparison of the katokatz, wet mount, and formol. Formalinether sedimentation mgl is a wellknown technique for the examination of faeces for parasites, but some recent reports have indicated that its. Since the introduction of ritchies method in 1948, many laboratories have chosen the formalin ether concentration procedure for parasitological exams in fecal specimens.
The formol ethyl acetate sedimentation concentration technique is preferred by many clinical microbiology laboratories for the detection of parasites in stool samples. A comparison of a modified formalinether concentration technique with a commercial feconomics method and its two modifications in the investigation of blastocystis sp. However, taking into account the acknowledged dangers caused by exposure to ether and formaldehyde, research was initiated to develop techniques as efficient as ritchies method, 14. Formol ether concentration method in the diagnosis of active schistosoma mansoni in patients with detectable iha.
Formal ether sedimentation stool concentration technique. Comparison of routine and concentration techniques on. Links to pubmed are also available for selected references. Workers and seafarers will adapt the formalin ether concentration technique fect. A selfadministered questionnaire was used to record kap of students against intestinal parasitic infection. Therefore, the aim of this study was the evaluation performance of diagnostic methods of intestinal parasitosis in school age children in ethiopia. The sedimentation technique had low sensitivity and is relatively timeconsuming. Total 126 students gave stool samples which were analysed by formol ether concentration technique. Thus, this study aimed at evaluating a formol acetone concentration method in comparison with the conventional direct iodine preparation and formol ether concentration methods. Intestinal parasite assessment was done by direct smear technique and formol ether concentration methods. Further observations on the formol ether concentration technique for faecal parasites. Under diagnosis of intestinal schistosomiasis in a. Pdf comparison of routine and concentration techniques. The overall prevalence of intestinal helminths was 9.
Comparison of the katokatz, wet mount, and formolether concentration diagnostic techniques for intestinal helminth infections in ethiopia. A total of five species of intestinal helminth parasites were encountered during the study, ascaris lumbricoides had the highest percentage of 49 32. The modified katokatz technique was performed to estimate the parasitic burden, expressed in the number of protozoa per gram of stool. The present study was a crosssectional study which comprised of 127 students who were selected by stratified random sampling. Mohammad a al mofarreh 1, ahmad m al akwaa 2, sayed w hasan 1, ibrahim a al mofleh 2 1 dr. Al mofarreh polyclinic, kkuh, riyadh, saudi arabia 2 department of medicine, kkuh, riyadh, saudi arabia click here for correspondence address. Remove an equal volume of sediment with an applicator stick, mix with albumen on. Application of pcrbased tools to explore strongyloides. Formalin ether concentration technique laboratory stack. In particular, the formalin ether sedimentation technique 406th medical general laboratory mgl, first reported by ritchie 1948, is commonly used for detecting helminth eggs, larvae, and. The formolether concentration technique for intestinal parasites. Samples were collected from retail chicken shops and examined for helminthes parasites. Comparison formalinethyl ether sedimentation, formalin.
Diagnostic techniques in medical parasitology the biology of. The children were categorized into three age groups 68 represent 41. Jun 11, 2019 this study was aimed to assess the prevalence and associated factors of parasitic infections among patients. The modified ridleyallen formol ether concentration technique is the method of choice for routine use by most clinical laboratories and is the method on which most commercial kits are based. The number of parasitic forms of both protozoan andor helminthic parasites in fecal specimens is often too low to be observed microscopically in direct wet mounts or in stained smear preparation.
This resource was developed by charles adjei osei of the kwame nkrumah university of. Prevalence of intestinal parasitic infection among. Hemoglobin concentration was analyzed using cyanmethaemoglobin method. Soil transmitted helminths among primary school children. Aug 17, 2018 a crosssectional study was carried out among 562 psac 15 years old in 6 counties of hoima district using expanded program on immunization epi method. Under the republic act 10022 of the department of health, the preemployment stool exam of overseas filipino workers and seafarers will adapt the formalin ether concentration technique fect. Based on the findings in this study we conclude that the formol ethyl acetate sedimentation concentration technique, using parasep midi faecal parasite concentrators, offers no advantages compared to katokatz and hatching in lowintensity s. This procedure entails filtration of a faecal suspension in 10% formalin in water, solvent extraction of debris and fat, followed by centrifugation. Evaluation and modification of the formalinether sedimentation. Formol ether concentration techniques using an applicator stick, about 1g of the feces mixed with normal saline was put in a screwcap bottle containing 4ml of 10% water. Mix the contents of the vial by shaking vigorously or vortexing for 30 seconds.
Six different gastrointestinal parasites were isolated and identified. The highest prevalence of infections was recorded among the age group of 19 22 years 21% in both sexes, and least infection rate in the age group of 27 30. Formalinethyl acetate sedimentation, and zinc sulfate. This video demonstrates both the sedimentation formol ether and flotation zinc sulfate methods of stool feces concentration. Pdf the formolether concentration technique for intestinal. In simple salt flotation method, about 1gm of feces were emulsified with 34 ml of saturated salt solution in a 20ml conical glass test tube. The use of ether has since been substituted with ethylacetate, without any change in sensitivity erdman, 1981, to become the fea concentration technique used in this study. Ridley fromthe hospitalfor tropical diseases, stpancras way,london thesimplified version ofritchies formol ether technique for the concentration offaecal cysts, ova, and larvae ridley and hawgood, 1956, which is no. Single stool sample was processed for direct, formol ether concentration. Technical methods further observations on the formol ether concentration technique for faecal parasites a.
The value of formolether concentration of faecal cysts and ova. This method increases the sensivity as compare to direct wet mount with dobell iodine or normal saline. The formolether concentration technique for intestinal. The formalether concentration technique was performed by adding 1 g of faeces to 5 ml of formalin 10%, which was emulsified and strained, and the filtrate centrifuged for 2 min at 3000 rpm. Time delays between patient and laboratory selectively affect. A modified zinc sulfate flotation technique using formalinized fecal specimens fznso4 was compared to the formalin ether fe concentration method for the laboratory diagnosis of intestinal parasites. The endemic countries are in a diagnostic dilemma concerning schistosoma japonicum with increasing difficulties in diagnosing the infected individuals. Since first being identified as a wildlife reservoir of bovine tuberculosis in 1974 there has been an increased research focus into the behaviour of these ecologically important mammals in the republic of ireland roi. We assessed the diagnostic accuracy of the katokatz method and the formalin ether concentration technique fect for c. This study was aimed to assess the prevalence and associated factors of parasitic infections among patients. Comparison of direct smear and formol ether concentration techniques in diagnosing intestinal parasites. Jcdr concentration techniques, simple salt floatation, zinc.
Pdf a modified formalinether concentration technique for. Knowledge, attitude and practice kap assessment of. For further confirmation, formol ether concentration technique was performed. The proportion of overall infected samples was 31%. Thereafter, the feces was sieved, and the sieved suspension collected in a beaker. I think mike cruickshanks new profit maximiser service is going to be one of the big hits and i can say hand on heart, if you follow the instructions, and just get involved, you will make a lot of money over the coming weeks, months and years. Gastro intestinal helminths parasites of local chickens. A cross sectional study was conducted on 364 patients, attending shahura health center, northwest ethiopia. Further observations on the formolether concentration technique for faecal parasites. Ether concentration technique in diagnosis of intestinal parasites.
Miniflotac, an innovative direct diagnostic technique for. A cross sectional study was conducted from may to june 20. Three techniques were applied on all samples which included direct microscopic examination by using saline and iodine preparations and the microscopic examination after using two concentration techniques which included simple salt flotation technique and formal ether concentration technique. Further observations on the formolether concentration. Get a printable copy pdf file of the complete article 319k. Formolether concentration method in the diagnosis of. Different studies showed that formol ether concentration technique fec is more sensitive than the conventional direct wet mount microscopy. The present study was aimed to validate a new copromicroscopic technique, the miniflotac and to compare its diagnostic efficiency and sensitivity with four other copromicroscopic techniques.
International journal of pharmacy and biological sciences vijay prakash singh et al. Formal ether sedimentation technique for the concentration of. When ranked by proportion, parasite loads were found as follows. Formalin ether or formalin ethyl acetate method is the recommended concentration procedures. Schistosoma mansoni was the dominant parasites as detected by both direct wet mount and formol ether concentration technique with 4452 84. Although several diagnostic methods such as katokatz and formol ether concentration fec techniques are available, direct wet mount is the commonly used as a reliable diagnosis method for the diagnosis of intestinal parasitic infections generally in africa and particularly in ethiopia 10.
Formal ether sedimentation technique for the concentration of stool. A modified formalin ether concentration technique for diagnosis of human strongyloidiasis article pdf available in the korean journal of parasitology 516. The simplified version of ritchies formolether tech nique for the. Comparison of the flotac400 dual technique and the. The european badger meles meles is irelands largest terrestrial carnivore. More than 10 faecal examination techniques are known, including direct smear ds andor concentration tada et al. Knowledge attitudes and practices of grade three primary. Pdf we compared the efficacy and applicability of a modified formalinether concentration technique mfect to the conventional fect cfect and the. The first page of the pdf of this article appears above. Evaluation performance of diagnostic methods of intestinal. This six minute video presents the formol ether concentration technique used to examine fecal parasites.
Formalin ether concentration technique is a method use to concentrate parasitic elements through sedimentation to enhance recovery. The simplified version of ritchies formolether tech nique for the concentration of faecal cysts, ova, and larvae ridley and hawgood, 1956, which is no less. Transmission of schistosoma mansoni in yachi areas. The formol ether concentration technique on the other hand is timeconsuming, and requires several materials. Vernier measurements should be taken if an unusual parasite is identified or if a confirmation is required. We compared the efficacy and applicability of a modified formalinether concentration technique mfect to the conventional fect cfect and the agar plate culture apc method for the detection of strongyloides stercoralis larvae.
The fec study on a total of 80 100% elementary students showed that 12 15% sample had the sth. Among these concentration techniques,the two most commonly used stool concentration techniques. Of the total of three hundred and thirty four 334 subjects, one hundred and fifty two 152 were females and one hundred and eighty two 182 were males. Responsibilities function activities laboratory technician perform the formalin ether concentration technique blinded to the results of the reference tests. For this purpose, we used 600 human fecal specimens collected in an endemic area of southern thailand. The sedimentation technique used at cdc is the formalinethyl acetate technique, a diphasic sedimentation technique that avoids the problems of flammability of ether, and which can be used with specimens preserved in formalin, mif or saf. Four layers formed after the second centrifugation of the fact improved diagnosis of strongyloides stercoralis using recombinant antigenbased. Prevalence of human intestinal helminth parasites among. Bukumbi for intestinal parasitic infections with three diagnostic methods. Filter the emulsion through fine mesh gauze into a conical centrifuge tube. If washed sediment is mainly mucous, place 1 drop of mayers albumen on 2 slides.
The formol ether concentration fec technique ridley and hawgood, 1956 is also widely used and has the advantages of fixing the parasites, thus rendering the samples noninfectious, as well as preserving many types of cysts, which the kk technique does not cheesbrough, 2005. The most common intestinal nematodes of dogs are toxocara canis, hookworm and trichuris vulpis. Out of the 76 persons who were both reference standard and sedimentation technique negative, there were 18 only positive with iha, 5 only with katokatz and 4 with a positive hatching test only. The technologist will prepare a direct smear from the remaining sediment. Request pdf the formolether concentration technique for intestinal. Prevalence of intestinal helminth infections among school children. Formalinether sedimentation concentration definition of. Prevalence of intestinal helminth infections among school. Additionally, we included another flotation method, namely, the saltsugar flotation ssf technique as an alternative to fea. Oct 29, 20 clonorchiasis is a chronic neglected disease caused by a liver fluke, clonorchis sinensis.
The study was designed at state elementary school 27 peusangan, bireuen. Stool samples from children were examined using the formol ether concentration technique for sth egg detection. The two most commonly used stool concentration techniques. Most types of worm eggs round worms, tapeworms, schistosomes, and other fluke eggs, larvae, and protozoan cysts may be recovered by this method. However knowledge, attitudes and practices kap of this target age group 810 years in relation to schistosomiasis, soil transmitted helminthiasis sths and malaria is not known at a time when integrated plasmodium helminth. Therefore, the employment of fec techniques as a confirmatory test in routine laboratory examination of stool will significantly aid in accurate determination and management of parasitic infections. Centrifuge time should be calculated after centrifuge reaches full speed. For each stool specimen collected formol ether concentration technique was performed.
Prevalence, intensity and factors associated with soil. Schistosome species lay only few numbers of eggs and only twothird of the eggs are excreted intermittently with stool, making single wet. Pdf a modified formalinether concentration technique. We compared the efficacy and applicability of a modified formalin ether concentration technique mfect to the conventional fect cfect and the agar plate culture apc method for the detection of strongyloides stercoralis larvae. Free fulltext pdf articles from hundreds of disciplines, all in one place miniflotac, an innovative direct diagnostic technique for intestinal parasitic infections. The formalinether concentration technique is typically conducted in reference laboratories for the concurrent diagnosis of helminth and intestinal protozoa. Formal ether sedimentation technique for the concentration. Next another 4 ml of 10% vv formol water was added and mixed well by shaking. Principle of formal ether formalinethyl aceatate sedimentation technique. A bayesian approach was used to evaluate the diagnostic performance of the evaluation tool. Comparison of formalinethyl ether sedimentation, formalinethyl. Chemotherapy is the mainstay of control and treatment efficacy is usually determined by microscopic examination of fecal samples. A total of 120 stool samples were collected, 60 male and 60 female from fepeaury to april 2014. Laboratory stack technical laboratory procedure guides for clinical laboratory scientists and students including the latest technology and.
Get a printable copy pdf file of the complete article 319k, or click on a page image below. The sedimentation technique processes a relatively large stool sample and is thus theoretically more sensitive than alternative techniques. Pdf further observations on the formolether concentration. Hemoglobin concentration of intestinal parasites infested. Formolether concentration method in the diagnosis of active. A comparison of a modified formalin ether concentration technique with a commercial feconomics method and its two modifications in the investigation of blastocystis sp. Pdf a comparison of a modified formalinether concentration. Laboratory procedures for stool examination laboratory. The formolethyl acetate sedimentation concentration technique subsequently termed sedimentation technique is one of the methods recommended in the clinical and laboratory standards institute formerly nccls guidelines and the cumitech guidelines for recovery of parasites from the intestinal tract.